Studies related to Pharmacokinetics
Studies related to Pharmacokinetics
PXB-mouse studies can be conducted using the same dosing and material sampling methods as other in vivo studies. The in-house study service of PhoenixBio consists of conducting the in-life phase trials and the collection of samples which are then sent to the client for evaluation. Upon request we also perform chemical analysis of the samples. PhoenixBio also offers an additional service which includes the measurement of human Albumin levels within the mouse serum, expression and activity of metabolic enzymes and blood biochemical measurements.
Dosing of Test-Compounds
Dosing Route
Oral, I.V, I.P, S.C.
Similar to other standard in vivo experiments, the compounds can be administered using all valid dosing routes
Sampling
Blood
Blood samples can be extracted on given time points using standard methods and techniques common to other in vivo experiments.
Bile
Bile can be collected continuously through cannulation or using standard methods common to other in vivo experiments.
Feces and Urine
Similar to other in vivo experiments, feces and urine can be collected using the metabolic cage.
Liver and other Organs
Organs are harvested upon completion of the study.
Standard Measurements
Concentration of human Albumin (h-Alb Value)
The human Albumin concentration in mouse serum is measured because this value correlates well with the replacement ratio of human hepatocytes in the mouse liver and can be used as an index of replacement.
Human metabolic enzymes
We measure the amount of human CYPs and mRNA and protein expression of Phase II conjugating enzymes and enzyme activity together with the P450 content.
Examination of Blood chemistry
We measure standard blood chemistry values such as GOT, GPT, γ-GTP, AG, TCHO, etc.
Examples of Studies using PXB-mice
DMPK (Drug Metabolisms and Pharmacokinetics) related
| Metabolic Profiling |
| Enzyme Induction/Inhibition |
| ADME Studies |
| Drug-Drug Interaction Studies (metabolic enzymes, transporters, MBI etc.) |
| Bile Excretion Studies |
| Human PK Evaluation Studies |
| Measurement of Protein Binding |
| Bioavailability Evaluation |
Pharmacological Studies
| Efficacy Evaluation of Liver Regeneration Drugs |
Drug Safety Studies (Toxicicological Studies)
| Peroxisomal Proliferation Study |
| Hepatitic Toxicity Study (Acetaminophen, Flutamide) |
| Study on Hepatic Toxicity attributable to Covalent Binding |
| Toxicogenomics Study |
Case list
- What are PXB-mice?
- Studies related to Pharmacokinetics
- Studies Related to Hepatitis Virus Research
- Studies using Radioisotopes and the GLP Standard
